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Recombinant Mouse Lipocalin-2/NGAL/LCN2 Protein (RP01064)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Mouse Lipocalin-2/NGAL/LCN2 Protein
Catalog No.RP01064
DescriptionRecombinant Mouse Lipocalin-2/NGAL  Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln21-Asn200) of mouse Lipocalin-2/NGAL  (Accession #NP_032517.1) fused with an 8×His tag at the C-terminus.
Bio-ActivityMeasured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant mouse Lipocalin-2. Recombinant mouse Lipocalin-2 can bind >47.7 μM of Fe(DHBA)3.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesMouse
Calculated MW21.71 kDa
SynonymNGAL; LCN2; Lipocalin-2; 24p3; MSFI; LCN2
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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