제품 > 재조합 단백질

Recombinant Mouse MFG-E8 Protein (RP01490)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Mouse MFG-E8 Protein
Catalog No.RP01490
DescriptionRecombinant Mouse MFG-E8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala23-Cys463) of mouse MFGE8/BA46 (Accession #NM_008594.2) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 25.33-101.30 ng/mL, corresponding to a specific activity of 9.87×103 ~3.95×104
Purity> 95% by SDS-PAGE.
Endotoxin<0.1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesMouse
Calculated MW49.84 kDa
MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to the phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.
SynonymP47; MP47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin; MFGE8
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RP01490? Please let us know so that we can cite the reference in this datasheet.

용량을 선택하세요

대량구매 문의하기

문의하기