Overview
| Product name | Recombinant Mouse Macrophage migration inhibitory factor/MIF Protein |
|---|
| Catalog No. | RP01829 |
|---|
| Description | Recombinant Mouse Macrophage migration inhibitory factor/MIF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro2-Ala115) of mouse Macrophage migration inhibitory factor/MIF (Accession #NP_034928.1) |
|---|
| Purity | > 95% by SDS-PAGE. |
|---|
| Endotoxin | < 0.1 EU/μg |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM Tris 150mM NaCl pH8.0. |
|---|
| Species | Mouse |
|---|
| Calculated MW | 12.37 kDa |
|---|
Background
MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4 - 7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity (12).
Product information
| Synonym | GIF; DER6; Glif；Macrophage migration inhibitory factor；MIF |
|---|
| Expression Host | E. coli |
|---|
| Tag | NO-Tag |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
