| Product name | Recombinant Mouse Osteopontin/SPP-1 Protein |
|---|---|
| Catalog No. | RP02806 |
| Description | Recombinant Mouse Osteopontin/SPP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu17-Asn294) of mouse Osteopontin/SPP1 (Accession #NP_033289.2) fused with a 6×His tag at the C-terminus. |
| Bio-Activity | Measured by the ability of the immobilized protein to support the adhesion of B16 cells.When 5×104 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour |
| Purity | > 95% as determined by HPLC |
| Endotoxin | <1EU/μg |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Mouse |
| Calculated MW | 31.59 kDa |
| Synonym | Spp1; Eta-1; Op; Spp-1; Osteopontin; 2AR; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Minopontin; Secreted phosphoprotein 1; SPP-1 |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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