Overview
| Product name | Recombinant Mouse PVR/CD155 Protein |
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| Catalog No. | RP01551 |
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| Description | Recombinant Mouse PVR/CD155 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp29-Leu348) of mouse PVR/CD155 (Accession #NP_081790.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse CD155 at 5 μg/mL (100 μL/well) can bind Mouse TIGIT with a linear range of 1.2-17.8 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | <0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 35.62 kDa |
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Background
The recombinant human PVR consists of 323 amino acids with a molecular weight of 35.1 kDa.CD155 is also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5) It is a type I transmembrane single-span glycoprotein belonging to the nectins and nectin-like (Necl) subfamily. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system.Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
Product information
| Synonym | CD155; HVED; Necl-5; NECL5; PVS; TAGE4; PVR; CD155; HVED; Necl-5; NECL5; PVS; TAGE4; PVR |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
