Overview
| Product name | Recombinant Mouse Periostin/PN/POSTN Protein |
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| Catalog No. | RP01771 |
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| Description | Recombinant Mouse Periostin/PN/POSTN Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn24-Ile653) of mouse Periostin/PN/POSTN (Accession #NP_056599.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >13% cell adhesion.
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
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| Endotoxin | < 0.001EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 70.64 kDa |
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Background
Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis.Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.
Product information
| Synonym | PN; PLF; Osf2; OSF-2; A630052E07Rik；POSTN |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
