Overview
| Product name | Recombinant Mouse Pleiotrophin/PTN Protein |
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| Catalog No. | RP00791 |
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| Description | Recombinant Mouse Pleiotrophin/PTN Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Gly33-Asp168) of mouse Pleiotrophin/PTN (Accession #P63089) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cerebral cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been pre-coated with 100 μL/well of Recombinant Mouse Pleiotrophin/PTN at 3-8 μg/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation. |
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| Species | Mouse |
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| Calculated MW | 16.1 kDa |
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Background
Pleiotrophin (PTN) is a secreted, strongly heparinbinding, developmentally regulated cytokine. PTN is a highlyconserved protein ， Human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequenceidentity or greater. PTN and midkine share 50% amino acid (aa) sequence identity, share some functions, andconstitute a family. During development, PTN is involved in development of brain, bone, and organsundergoing branching morphogenesis. PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates. Increased expression of PTN is correlatedwith neuronal development or stresses such as brain ischemia and Parkinson’s disease.
Product information
| Synonym | Pleiotrophin; PTN; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Osteoblast-specific factor 1; OSF-1; |
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| Expression Host | HEK293 cells |
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| Tag | C-6×His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
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* For research use only. Not for therapeutic or diagnostic purposes.
