Overview
| Product name | Recombinant Mouse/Rat Mature TGF-beta 1 Protein |
|---|
| Catalog No. | RP00671 |
|---|
| Description | Recombinant Mouse TGF-beta 1/TGFB1 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ala279-Ser390) of mouse TGF-beta 1/TGFB1 (Accession #P04202). |
|---|
| Bio-Activity | Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 Mouse T cells. The ED50 for this effect is 0.054-0.214 ng/mL, corresponding to a specific activity of 4.7×106~1.85×107 units/mg.
|
|---|
| Purity | > 95% by SDS-PAGE. |
|---|
| Endotoxin | < 0.01 EU/μg |
|---|
| Formulation | Lyophilized from a 0.2 μm filtered solution of 50 mM Glycine, 150 mM NaCl, pH3.5. Contact us for customized product form or formulation. |
|---|
| Species | Mouse |
|---|
| Calculated MW | 12.81 kDa |
|---|
Background
Transforming growth factor beta 1 (TGFβ1) is the prototype of a growing superfamily of peptide growth factorsand plays a prominent role in a variety of cellular processes, including cell-cycle progression, celldifferentiation, reproductivefunction, development, motility, adhesion, neuronalgrowth, bonemorphogenesis, wound healing, and immune surveillance. TGF- β 1, TGF- β 2 and TGF- β 3 signal via the sameheteromeric receptor complex, consisting of a ligand binding TGF- β receptor type II (T β R-II), and a TGF- βreceptor type I (T β R-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF- βexpression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.
Product information
| Synonym | TGF-beta1; Tgfb; Tgfb-1; TGFbeta1; CED; DPD1; LAP; TGFB; TGFbeta; TGFB1 |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | No tag |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
