Overview
| Product name | Recombinant Mouse/Rat Mature TGF-beta 2 Protein |
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| Catalog No. | RP00672 |
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| Description | Recombinant Mouse TGF-beta 2/TGFB2 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ala303-Ser414) of mouse TGF-beta 2/TGFB2 (Accession #P27090). |
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| Bio-Activity | Recombinant Mouse/Rat Mature TGF-beta 2 Protein inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.062-0.248 ng/mL, corresponding to a specific activity of 4.03×106~1.61×107 units/mg.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.Contact us for customized product form or formulation. |
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| Species | Mouse/Rat |
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| Calculated MW | 12.69 kDa |
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Background
Transforming growth factor beta 2 (TGF- β 2) is a member of TGF-beta superfamily that shares a characteristiccysteine knot structure. Mice with TGF- β 2 gene deletion show defects in development of cardiac, lung, craniofacial, limb, spinal column, eye, inner ear and urogenital systems. All TGF-β isoforms signal via the sameheteromeric receptor complex, consisting of a ligand binding TGF- β receptor type II (T β R-II), and a TGF- βreceptor type I (T β R-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF- βexpression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.
Product information
| Synonym | TGFB2; BSC-1 cell growth inhibitor; Cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; Polyergin; TGF-beta2; TGF-beta-2; transforming growth factor beta-2 |
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| Expression Host | HEK293 cells |
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| Tag | No tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
