Overview
Product name | Recombinant Mouse Serpin F1/PEDF Protein |
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Catalog No. | RP01582 |
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Description | Recombinant Mouse Serpin F1/PEDF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln20-Thr417) of mouse Serpin F1 (Accession #NP_035470.3) fused with a 6×His tag at the C-terminus. |
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Purity | > 95% by SDS-PAGE. |
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Endotoxin | <0.1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Mouse |
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Calculated MW | 45.13 kDa |
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Background
The protein is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.
Product information
Synonym | EPC-1; OI12; OI6; PEDF; PIG35; SerpinF1 |
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Expression Host | HEK293 cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.