Overview
Product name | Recombinant Mouse Serum albumin/ALB Protein |
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Catalog No. | RP02805 |
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Description | Recombinant Mouse Serum albumin/ALB Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu25-Ala608) of mouse Serum albumin/ALB (Accession #NP_033784.2) fused with a 6×His tag at the C-terminus. |
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Bio-Activity | Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 54.53 nM as determined in SPR assay (Biacore T200).
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Purity | > 95% as determined by HPLC |
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Endotoxin | <1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Mouse |
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Calculated MW | 70.19 kDa |
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Background
Albumins are multifunctional proteins present in the blood serum of animals. They can bind and transport a wide variety of ligands which they accommodate due to their conformational flexibility. Serum albumins are highly conserved both in amino acid sequence and three-dimensional structure. Several mammalian and avian serum albumins (SAs) are also allergens. Sensitization to one of the SAs coupled with the high degree of conservation between SAs may result in cross-reactive antibodies in allergic individuals.
Product information
Synonym | ALB; HSA; PRO0883; PRO0903; PRO1341; albumin; Albumin ; Alb |
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Expression Host | HEK293 cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.