Overview
| Product name | Recombinant Mouse TFPI Protein |
|---|
| Catalog No. | RP01667 |
|---|
| Description | Recombinant Mouse TFPI Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu 29-Asn306) of mouse TFPI (Accession #NP_035706.1) fused with and a 6×His tag at the C-terminus. |
|---|
| Bio-Activity | Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2(RD, Catalog # ES002). The IC50 value is <0.26 nM, as measured under the described conditions.
|
|---|
| Purity | > 97% by SDS-PAGE. |
|---|
| Endotoxin | <0.1EU/μg |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
|---|
| Species | Mouse |
|---|
| Calculated MW | 32.53 kDa |
|---|
Background
Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation.
Product information
| Synonym | EPI; LACI; A630013F22Rik；TFPI |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-6His |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
