| Product name | Recombinant Mouse TNFRSF1A/TNF-R1/CD120a Protein |
|---|---|
| Catalog No. | RP01078 |
| Description | Recombinant Mouse TNFRSF1A/TNF-R1/CD120a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ala212) of mouse TNFR1/CD120a/TNFRSF1A (Accession #NP_035739.2) fused with an Fc, 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse TNF-alpha Protein at 0.5 μg/mL (100 μL/well) can bind Mouse TNFR1 with a linear range of 0.06-15.7 ng/mL. 2.Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 75-300 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-α. |
| Purity | > 97% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Species | Mouse |
| Calculated MW | 49.98 kDa |
| Synonym | CD120a; FPF; p55; p55-R; TNF-alphaR1; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNFalpha-R1; TNFAR; Tnfr-2; Tnfr1; TNFR60; TNFRI; TNFRp55; TNFRSF1A; CD120a; FPF; p55; p55-R; TNF-alphaR1; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNFalpha-R1; TNFAR; Tnfr-2; Tnfr1; TNFR60; TNFRI; TNFRp55; TNFRSF1A |
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| Expression Host | HEK293 cells |
| Tag | C-hFc&His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
