Overview
| Product name | Recombinant Mouse TNFSF12/TWEAK Protein |
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| Catalog No. | RP02821 |
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| Description | Recombinant Mouse TNFSF12/TWEAK Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Arg105-His249) of mouse TNFSF12/TWEAK (Accession #NP_035744.1) fused with a raFc tag at the C-terminus. |
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| Bio-Activity | Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-50 ng/mL.
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| Purity | > 90% by SDS-PAGE. |
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| Endotoxin | <1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 43.5 kDa |
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Background
TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.
Product information
| Synonym | TNFSF12; APO3L; DR3LG; TNLG4A; TWEAK |
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| Expression Host | HEK293 Cells |
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| Tag | C-raFc |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
