Overview
| Product name | Recombinant Mouse TNFSF9/4-1BB Ligand Protein |
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| Catalog No. | RP00766 |
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| Description | Recombinant Mouse TNFSF9/4-1BB Ligand Protein is produced by Human cells expression system. The target protein is expressed with sequence (Arg104-Glu309) of mouse TNFSF9/4-1BB Ligand (Accession #P41274) fused with aHis tag at the N-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse TNFSF9(Catalog: RP00766) at 5μg/mL (100 μL/well) can bind Mouse TNFRSF9/4-1BB/CD137
(Catalog: RP01560) with a linear range of 0.01-1.41 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation. |
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| Species | Mouse |
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| Calculated MW | 23.80 kDa |
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Background
Tumor necrosis factor ligand superfamily member 9, also known as 4-1BBL, is a member of the the tumornecrosis factor family. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain.The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but sharesonly low aa sequence homology (14-16%). 4-1BBL is predominantly expressed on activated antigen presentingcells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and Blymphoma cell lines. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting Tlymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses inCD8 T cells, and is thought to be involved in T cell-tumor cell interaction.
Product information
| Synonym | Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; Tnfsf9; Cd137l; Cd157l; Ly63l; TNFSF9 |
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| Expression Host | HEK293 cells |
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| Tag | N-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
