Overview
Product name | Recombinant Mouse TREM2 Protein |
---|
Catalog No. | RP01558 |
---|
Description | Recombinant Mouse TREM2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu19-Ser171) of mouse TREM-2 (Accession #NP_112544.1) fused with a 6×His tag at the C-terminus. |
---|
Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse TREM-2 Protein at 1 μg/mL (100 μL/well) can bind TREM-2 Rabbit pAb with a linear range of 0.06-11.7 ng/mL.
|
---|
Purity | > 90% by SDS-PAGE. |
---|
Endotoxin | Please contact us for more information. |
---|
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
---|
Species | Mouse |
---|
Calculated MW | 17.65 kDa |
---|
Background
Triggering Receptor Expressed on Myeloid cells 2 (TREM2)is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 and inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes.It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria
Product information
Synonym | TREM-2; Trem2a; Trem2b; Trem2c; TREM2 |
---|
Expression Host | HEK293 cells |
---|
Tag | C-His |
---|
Swiss-Prot | |
---|
Gene ID | |
---|
Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
---|
Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
---|
* For research use only. Not for therapeutic or diagnostic purposes.