Overview
| Product name | Recombinant Peptostreptococcusmagnus Protein L Protein |
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| Catalog No. | RPT0014 |
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| Description | Recombinant Peptostreptococcusmagnus Protein L Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val103-Glu408) of Peptostreptococcusmagnus Protein L (Accession #) fused with and 6×His tag at the N-terminus. |
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.01 EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Peptostreptococcusmagnus |
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| Calculated MW | 34.82 kDa |
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Background
Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG, IgM, IgA, IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
Product information
| Synonym | RPL; Protein L |
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| Expression Host | HEK293 cells |
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| Tag | N-His |
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| Swiss-Prot | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
