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Recombinant Rat 5'-Nucleotidase/NT5E/CD73 Protein (RP00570)

Datasheet

Overview

Product nameRecombinant Rat 5'-Nucleotidase/NT5E/CD73 Protein
Catalog No.RP00570
DescriptionRecombinant Rat 5'-Nucleotidase/NT5E/CD73  Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Trp29-Lys549) of rat 5'-Nucleotidase/NT5E/CD73  (Accession #P21588) fused with a 6×His tag at the C-terminus.
Purity> 90% by SDS-PAGE.
Endotoxin< 1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation.
SpeciesRat
Calculated MW59 kDa
CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosylphophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymesresponsible for the production of extracellular adenosine, a signaling molecule that is involved in responses toinflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeablenucleosides.
SynonymCD73; E5NT; Nucleotidase; NT5E; ecto-5'-nucleotidase
Expression HostHEK293 cells
TagC-6×His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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