Overview
| Product name | Recombinant Rat B7-H1/PD-L1/CD274 Protein |
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| Catalog No. | RP01568 |
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| Description | Recombinant Rat B7-H1/PD-L1/CD274 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala18-Thr238) of rat B7-H1/PD-L1/CD274 (Accession #NP_001178883.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Rat PD-L1 (Catalog: RP01568) at 10 μg/mL (100 μL/well) can bind Biotinylated Rat PD-1 with a linear range of 0.0195-2.16 μg/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | <0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Rat |
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| Calculated MW | 25.90 kDa |
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Background
This protein is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation.
Product information
| Synonym | Pdcd1lg1; RGD1566211; CD274 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
