Overview
| Product name | Recombinant Rat CSF-1/M-CSF Protein |
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| Catalog No. | RP01847 |
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| Description | Recombinant Rat CSF-1/M-CSF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ser255) of Rat CSF-1/M-CSF (Accession #NP_076471.3 ) fused with a His tag at the C-terminus. |
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| Bio-Activity | Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 5.63-22.51 ng/mL, corresponding to a specific activity of 4.44×104~1.78×105 units/mg.
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| Endotoxin | <0.1EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Rat |
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| Calculated MW | 28.50 kDa |
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Background
Macrophage colony-stimulating factor (M-CSF), also known as colony-stimulating factor-1 (CSF-1), is a hemopoietic growth factor that regulates the survival, proliferation, differentiation and function of the cells of the mononuclear phagocyte lineage. M-CSF is produced by a variety of cell types and acts both locally and humorally in an autocrine and paracrine manner. Through differential mRNA splicing and posttranslational proteolytic processing and modification, M-CSF is expressed in three biologically-active isoforms: a secreted glycoprotein; a secreted proteoglycan; and a membrane spanning cell-surface glycoprotein.
Product information
| Synonym | Macrophage colony-stimulating factor 1; CSF-1; MCSF; Proteoglycan macrophage colony-stimulating factor; PG-M-CSF; Cleaved into: Processed macrophage colony-stimulating factor 1; Macrophage colony-stimulating factor 1 43 kDa subunit，Csf1; Csfm |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
