Overview
| Product name | Recombinant Rat Cystatin-C/CST3 Protein |
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| Catalog No. | RP01668 |
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| Description | Recombinant Rat Cystatin-C/CST3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly 21-Ala140) of rat Cystatin-C/CST3 (Accession #NP_036969.1) fused with and a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009). The IC50 value is <3.4 nM, under the described conditions.
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| Purity | > 92% by SDS-PAGE. |
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| Endotoxin | <0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM HEPES and 150 mM NaCl，pH 7.0 |
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| Species | Rat |
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| Calculated MW | 14.17 kDa |
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Background
Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate.
Product information
| Synonym | CYSC；Cystatin-C；CST3 |
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| Expression Host | HEK293 cells |
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| Tag | C-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
