Overview
| Product name | Recombinant Rat IL-4 Protein |
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| Catalog No. | RP01874 |
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| Description | Recombinant Rat IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys25-Ser147) of Rat IL-4 (Accession #NP_958427.1) fused with a His
at the C-terminus. |
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| Bio-Activity | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.65-2.6 μg/mL, corresponding to a specific activity of 3.85×102~1.54×103 units/mg.
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| Endotoxin | <0.01EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Rat |
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| Calculated MW | 14.60 kDa |
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Background
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
Product information
| Synonym | B-cell growth factor 1; B-cell IGG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
