Overview
| Product name | Recombinant Rat IL-5 Protein |
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| Catalog No. | RP01849 |
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| Description | Recombinant Rat IL-5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met20-Val132) of Rat IL-5 (Accession #NP_068606.1 ) fused with a His tag at the C-terminus. |
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| Bio-Activity | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.695-2.78 ng/mL, corresponding to a specific activity of 3.60×105~1.44×106 units/mg.
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| Endotoxin | <0.1EU/μgof the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Rat |
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| Calculated MW | 13.91 kDa |
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Background
Interleukin-5 (IL-5), which is produced primarily by type 2 T helper lymphocytes (Th2), is an eosinophil differentiation and activation factor. Antagonism of IL-5 activity is being explored as a potential treatment of a number of disease conditions associated with eosinophils in animal models. Interleukin-5 (IL-5) which was previously named T cell replacing factor, B cell growth and differentiation factor, colony forming unit growth stimulating factor and eosinophil differentiation factor is produced predominantly by T cells.
Product information
| Synonym | Interleukin-5; IL-5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF，Il5; Il-5 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
