Overview
Product name | Recombinant Rat IL-9 Protein |
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Catalog No. | RP01664 |
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Description | Recombinant Rat IL-9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln19-Ala144) of rat IL-9 (Accession #NP_001099217.1) fused with and a 6×His tag at the C-terminus. |
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Bio-Activity | Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is 8.02-32.1 ng/mL.
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Purity | > 97% by SDS-PAGE. |
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Endotoxin | <0.1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Rat |
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Calculated MW | 14.98 kDa |
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Background
IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases.
Product information
Synonym | IL9 |
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Expression Host | HEK293 cells |
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Tag | C-6His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.