| Product name | Recombinant Rat MMP-9 Protein |
|---|---|
| Catalog No. | RP01212 |
| Description | Recombinant Rat MMP-9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu35-Pro708) of rat MMP-9 (Accession #NP_038627.1.) fused with a 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured in a cell migration assay using Hela cells. 1 ng/mL of Recombinant Rat MMP-9 can effectively induce Hela cells migration. 2.Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >1600 pmoles/min/μg. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 10mM CaCl2, 150mM NaCl, pH 7.5.Contact us for customized product form or formulation. |
| Species | Rat |
| Calculated MW | 75.55 kDa |
| Synonym | 92 kDa gelatinase; 92 kDa type IV collagenase; GELB; MMP9; MMP-9; MMP9 |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
