Overview
| Product name | Recombinant Rat Thrombopoietin /THPO / TPO Protein |
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| Catalog No. | RP01901 |
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| Description | Recombinant Rat THPO / TPO / Thrombopoietin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser22-Ser326) of Rat THPO / TPO / Thrombopoietin (Accession #NP_112395.1) fused with a His tag at the C-terminus. |
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| Bio-Activity | Measured in a cell proliferation assay using M07e human megakaryocytic leukemic cells. The ED50 for this effect is 0.26-1.02 ng/mL, corresponding to a specific activity of 9.8×105~3.8×106 units/mg.
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| Endotoxin | <0.01EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Rat |
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| Calculated MW | 33.10 kDa |
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Background
Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c mpl. Defects in the Tpo Tpo R signaling pathway are associated with a variety of platelet disorders . Mature rat Tpo shares 68% and 81% aa sequence homology with human and mouse Tpo, respectively . It is an 80 85 kDa protein that consists of an N terminal domain with homology to Erythropoietin (Epo) and a C terminal domain that contains multiple N linked and O linked glycosylation sites. Tpo promotes the differentiation, proliferation, and maturation of megakaryocytes (MK) and their progenitors . Several other cytokines can also promote these functions but only in cooperation with Tpo . Notably, IL 3 independently induces MK development, although its effects are restricted to early in the MK lineage . Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation . These actions, in combination with direct effects on cardiomyocytes, can aid in the recovery of heart function following myocardial infarction . Tpo is cleaved by platelet derived thrombin following Arg191 within the C terminal domain and subsequently at other sites upon extended digestion . The C terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation . Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling .
Product information
| Synonym | Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
