| Product name | Recombinant Rat VEGF-A/VEGF164 Protein |
|---|---|
| Catalog No. | RP01323 |
| Description | Recombinant Rat VEGF-A/VEGF164 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala27-Arg190) of Rat VEGFA (Accession #NP_001274037.1) fused with an 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Rat VEGF164 at 1 μg/mL (100 μL/well) can bind Human KDR with a linear range of 0.03-3.6 ng/mL. 2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.02-0.10 ng/mL. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Rat |
| Calculated MW | 20.07 kDa |
| Synonym | MVCD1; VAS; vascular endothelial growth factor A; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VEGFA |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
