| Product name | Recombinant SARS-CoV-2 Spike RBD(B.1.640.2/IHU) Protein |
|---|---|
| Catalog No. | RP02104 |
| Description | Recombinant SARS-CoV-2(2019-nCoV) Spike RBD(B.1.640.2/IHU)-His Protein is produced by Expi293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y)) of SARS-COV-2(2019-nCoV) Spike RBD(B.1.640.2/IHU)-His (Accession #QHD43416.1) fused with His tag at the C-terminus. |
| Bio-Activity | Immobilized SARS-Cov-2 Spike RBD(B.1.640.2/IHU), His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3 ng/mL determined by ELISA. |
| Purity | >95% by SDS-PAGE;> 95% by HPLC |
| Endotoxin | < 1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | SARS-CoV-2 |
| Calculated MW | 40 kDa |
| Synonym | Envelope; SARS-CoV-2 Spike RBD (N501Y); Spike; Spike ECD; Spike RBD; Spike S1; Spike S2; Spike S2 ECD; S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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