Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD) Protein (RP01260MT)

Product name	Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD) Protein
Catalog No.	RP01260MT
Description	Recombinant SARS-CoV-2(2019-nCoV) S1+S2 ECD（S-ECD） Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence Val16-Glu1188 of SARS-COV-2(2019-nCoV) S1+S2 ECD（S-ECD） (Accession #QHD43416.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1+S2 ECD at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-3.03 ng/mL.
Purity	> 97% by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Species	SARS-CoV-2
Calculated MW	132.9kDa

Synonym	Envelope; SARS-CoV-2 Spike RBD (N501Y); Spike; Spike ECD; Spike RBD; Spike S1; Spike S2; Spike S2 ECD; S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein
Expression Host	HEK293 cells
Tag	C-His&Avi
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.