| Product name | Recombinant Serratia marcescens Nuclease |
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| Catalog No. | RPT0008LQ |
| Description | Recombinant Serratia marcescens Nuclease Protein is produced by Pichia expression system. The target protein is expressed with sequence (Asp22-Asn266) of serratia marcescens Nuclease (Accession #WP_015377376.1) fused with no additional amino acid. |
| Bio-Activity | 1.One unit (U) is defined as the amount of enzyme required to change the absorption value of △A260 by 1.0 (equivalent to complete digestion of 37 μg of salmon essence DNA into oligonucleotides) in 30 min at 37°C, pH 8.0 reaction conditions. The specific activity of Serratia marcescens nuclease is > 1000 U/μL. 2.Recombinant Serratia marcescens Nuclease (0.1U) can effectively degrade 5 μg plasmid DNA at 37℃ for 30 min. The reaction buffer is: 10 mM MgCl2, 0.1 mg/mL BSA, 50 mM Tris-HCl, pH8.5. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Formulation | Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl. |
| Species | Serratia marcescens |
| Calculated MW | 26.71 kDa |
| Synonym | Endonuclease; Nuclease; nucA; nuc |
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| Expression Host | Pichia |
| Tag | No tag |
| Swiss-Prot | |
| Gene ID |
| Storage | stable at -70℃.This product is stable at ≤ -20℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
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* For research use only. Not for therapeutic or diagnostic purposes.
