Overview
| Product name | Recombinant human FGF-23 Protein |
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| Catalog No. | RP01740 |
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| Description | Recombinant human FGF-23 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr25-Ile251) of human FGF-23/Fibroblast growth factor 23 (Accession #NP_065689.1) fused witha 6×His tag at the C-terminus. |
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| Purity | > 97% by SDS-PAGE. |
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| Endotoxin | < 0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM MOPS，150mM NaCl，5mM EDTA，2mM DTT, pH 7.4 |
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| Species | human |
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| Calculated MW | 26.17 kDa |
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Background
Fibroblast growth factor 23 (FGF23) is is an endocrine member of the family of FGFs and mainly produced in the bone and, upon secretion, forms a complex with a FGF receptor and coreceptor αKlotho. FGF23 can exert several endocrine functions, such as acting as a hormone on the kidney, stimulating phosphate excretion and suppressing formation of 1, 25(OH)2D3, active vitamin D. Moreover, it has paracrine activities on several cell types, including neutrophils and hepatocytes. FGF23 and phosphate have been revealed to be factors relevant in cancer. FGF23 is particularly significant for those forms of cancer primarily affecting bone (e.g., multiple myeloma) or characterized by bone metastasis.
Product information
| Synonym | ADHR; FGFN; HYPF; HFTC2; HPDR2; PHPTC |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
