| Product name | ABplex Human Cytokine 12-Plex Assay Kit |
|---|---|
| Catalog No. | RK04296 |
| Size | 96 T |
| Sample | 100 ul |
| Instrument | Microplate Reader |
| Detection | Colorimetric |
| Assay Time | 2 hours |
| Conjugate | Biotin |
| Assay Type | fluorometric |
| Sample type | 2 hours |
|---|---|
| Cross reacts with | Human |
| Product overview | The ABplex Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. |
| Intended use | This ABplex Human Cytokine 12-Plex Assay Kit ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ABplex Human Cytokine 12-Plex Assay Kit in the sample, this ABplex Human Cytokine 12-Plex Assay Kit ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus ABplex Human Cytokine 12-Plex Assay Kit concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Storage instructions | Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month. |
| Sensitivity | IL-1β:3.5pg/mL、IL-2:2.5pg/mL、IL-4:2.5pg/mL、IL-5:2.5pg/mL、IL-6:2.5pg/mL、IL-8:5pg/mL、IL-10:3.5mg/mL、IL-12p70:2.5pg/mL、IL-17A:5pg/mL、IFN-α:3.5pg/mL、IFN-γ:3.5pg/mL、TNF-α:2.5pg/mL |
| Standard curve range | IL-1β:3.5-5000pg/mL、IL-2:2.5-5000pg/mL、IL-4:2.5-5000pg/mL、IL-5:2.5-5000pg/mL、IL-6:2.5-5000pg/mL、IL-8:5-5000pg/mL、IL-10:3.5-5000pg/mL、IL-12p70:2.5-5000pg/mL、IL-17A:5-5000pg/mL、IFN-α:3.5-5000pg/mL、IFN-γ:3.5-5000pg/mL、TNF-α:2.5-5000pg/mL |
| Components | Microtiter Plate Enzyme Conjugate Standards Substrate A Substrate B Stop Solution Wash Solution Balance Solution |
| Safety notes | 1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal. 2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents. |
* This package insert must be read in its entirety before using this product.
* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using RK04296? Please let us know so that we can cite the reference in this datasheet.
