Human TNF-alpha ELISA Kit (RK00030)

Overview

Product name	Human TNF-alpha ELISA Kit
Catalog No.	RK00030
Size	96 T
Sample	100 ul
Instrument	Microplate Reader
Detection	Colorimetric
Assay Time	4 hours
Conjugate	Biotin
Assay Type	Sandwich ELISA

Additional information

Sample type	Serum, plasma and cell culture supernates
Cross reacts with	Human
Product overview	The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in Serum, plasma and cell culture supernates
Intended use	This Human TNF-alpha ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human TNF-alpha in the sample, this Human TNF-alpha ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human TNF-alpha concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructions	Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Sensitivity	6.9 pg/mL
Standard curve range	15.6-1000 pg/ml
Components	Microtiter Plate Enzyme Conjugate Standards Substrate A Substrate B Stop Solution Wash Solution Balance Solution
Safety notes	1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal. 2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

* This package insert must be read in its entirety before using this product.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Journal of medical virology

Application:

ELISA

IF:

6.8

Species:

Homo sapiens

PMID:

30701565

Title:

Imbalance in the ratio of CpG and polyG contributes to impaired interferon-α expression.

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Molecular immunology

Application:

ELISA

IF:

3.2

Species:

Homo sapiens

PMID:

31351411

Title:

Structure-based rational design of a novel chimeric PD1-NKG2D receptor for natural killer cells.

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Diabetes Research and Clinical Practice

Application:

ELISA

IF:

6.1

Species:

Homo sapiens

PMID:

31476346

Title:

Correlation between inflammatory markers and impaired circadian clock gene expression in type 2 diabetes mellitus.

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Geroscience

Application:

ELISA

IF:

5.3

Species:

Homo sapiens

PMID:

32418021

Title:

Correlations of Gut Microbiome, Serum Metabolome and Immune Factors in Insomnia

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Evid Based Complement Alternat Med

Application:

ELISA

IF:

Species:

Mus musculus

PMID:

32765635

Title:

Salvia miltiorrhiza Injection Alleviates LPS-Induced Acute Lung Injury by Adjusting the Balance of MMPs/TIMPs Ratio

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Biomed Res Int

Application:

ELISA

IF:

Species:

Mus musculus

PMID:

32775410

Title:

The Effects of Titanium Surfaces Modified with an Antimicrobial Peptide GL13K by Silanization on Polarization, Anti-Inflammatory, and Proinflammatory Properties of Macrophages

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Mol Immunol

Application:

ELISA

IF:

3.2

Species:

Homo sapiens

PMID:

33068832

Title:

VEGF165b augments NK92 cytolytic activity against human K562 leukemia cells by upregulating the levels of perforin and granzyme B via the VEGR1-PLC pathway

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Am J Chin Med

Application:

ELISA

IF:

4.8

Species:

Mus musculus

PMID:

32933311

Title:

Ginsenoside Rb1 Ameliorates Age-Related Myocardial Dysfunction by Regulating the NF-κ B Signaling Pathway

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Front Immunol

Application:

ELISA

IF:

5.7

Species:

Mus musculus

PMID:

33362784

Title:

Interferon Regulatory Factor 5 Mediates Lipopolysaccharide Induced Neuroinflammation

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Evid Based Complement Alternat Med

Application:

ELISA

IF:

Species:

Mus musculus

PMID:

33456488

Title:

Bupleurum chinense Polysaccharide Improves LPS-Induced Senescence of RAW264.7 Cells by Regulating the NF- κ B Signaling Pathway

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Reprod Toxicol

Application:

ELISA

IF:

3.3

Species:

Mus musculus

PMID:

32446930

Title:

The protective effects of pretreatment with resveratrol in cyclophosphamide-induced rat ovarian granulosa cell injury: In vitro study

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

World J Gastroenterol

Application:

ELISA

IF:

4.3

Species:

Homo sapiens

PMID:

33505141

Title:

Gegen Qinlian decoction enhances immunity and protects intestinal barrier function in colorectal cancer patients via gut microbiota

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Front Microbiol

Application:

ELISA

IF:

6.1

Species:

Homo sapiens

PMID:

34512565

Title:

Elevated Systemic and Intestinal Inflammatory Response Are Associated With Gut Microbiome Disorder After Cardiovascular Surgery

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Oxidative medicine and cellular longevity

Application:

ELISA

IF:

Species:

Homo sapiens

PMID:

36046683

Title:

Autophagy-Mediated Inflammatory Cytokine Secretion in Sporadic ALS Patient iPSC-Derived Astrocytes - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Microbiology spectrum

Application:

ELISA

IF:

3.7

Species:

Homo sapiens

PMID:

36190400

Title:

Multiomics Analysis Reveals Aberrant Metabolism and Immunity Linked Gut Microbiota with Insomnia

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

The Science of the total environment

Application:

ELISA

IF:

8.2

Species:

Homo sapiens

PMID:

34717999

Title:

Inflammatory and oxidative stress responses of healthy elders to solar-assisted large-scale cleaning system (SALSCS) and changes in ambient air pollution: A quasi-interventional study in Xi'an, China

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

eLife

Application:

ELISA

IF:

6.4

Species:

Mus musculus

PMID:

35972780

Title:

Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDME - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

biomedicine & pharmacotherapy

Application:

ELISA

IF:

6.9

Species:

Homo sapiens

PMID:

29775893

Title:

Puerarin prevents vascular endothelial injury through suppression of NF-κB activation in LPS-challenged human umbilical vein endothelial cells

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Mol Immunol

Application:

ELISA

IF:

3.2

Species:

Homo sapiens

PMID:

34252709

Title:

Naringenin inhibits pro?inflammatory cytokine production in macrophages through inducing MT1G to suppress the activation of NF?κB

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

International journal of oncology

Application:

ELISA

IF:

4.5

Species:

Homo sapiens

PMID:

36004469

Title:

Liproxstatin‑1 induces cell cycle arrest, apoptosis, and caspase‑3/GSDME‑dependent secondary pyroptosis in K562 cells

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Biology of reproduction

Application:

ELISA

IF:

3.1

Species:

Homo sapiens

PMID:

36721984

Title:

Extracellular vesicles contribute to EMT in Adenomyosis by inducing macrophage polarization

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Molecular immunology

Application:

ELISA

IF:

3.2

Species:

Homo sapiens

PMID:

36736195

Title:

Engagement of an optimized lentiviral vector enhances the expression and cytotoxicity of CAR in human NK cells

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Food research international (Ottawa, Ont.)

Application:

ELISA

IF:

Species:

Homo sapiens

PMID:

36461372

Title:

RNA-seq based elucidation of mechanism underlying the protective effect of Huangshui polysaccharide on intestinal barrier injury in Caco-2 cells

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Heliyon

Application:

ELISA

IF:

Species:

Mus musculus

PMID:

38034611

Title:

Inhibition of SIRT6 aggravates p53-mediated ferroptosis in acute lung injury in mice - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Journal of neuroinflammation

Application:

ELISA

IF:

9.6

Species:

Mus musculus

PMID:

37990234

Title:

Knockdown and inhibition of hippocampal GPR17 attenuates lipopolysaccharide-induced cognitive impairment in mice - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Frontiers in immunology

Application:

ELISA

IF:

5.7

Species:

Homo sapiens

PMID:

35812413

Title:

Aqueous Extract of Descuraniae Semen Attenuates Lipopolysaccharide-Induced Inflammation and Apoptosis by Regulating the Proteasomal Degradation and IRE1-Dependent Unfolded Protein Response in A549 Cells - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Journal of nanobiotechnology

Application:

ELISA

IF:

10.2

Species:

Homo sapiens, Mus musculus

PMID:

37968665

Title:

Mannose-doped metal-organic frameworks induce tumor cell pyroptosis via the PERK pathway - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Journal of neuroimmunology

Application:

ELISA

IF:

2.9

Species:

Homo sapiens

PMID:

37925900

Title:

CSF CXCL13 is elevated in patients with CIDP and may reflect higher disease activity

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Molecular and cellular endocrinology

Application:

ELISA

IF:

3.8

Species:

Homo sapiens

PMID:

37028587

Title:

Melatonin enhances anti-tumor immunity by targeting macrophages PD-L1 via exosomes derived from gastric cancer cells

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Molecular immunology

Application:

ELISA

IF:

3.2

Species:

Homo sapiens

PMID:

38128392

Title:

The SpyCatcher-SpyTag interaction mediates tunable anti-tumor cytotoxicity of NK cells

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Archives of dermatological research

Application:

ELISA

IF:

1.8

Species:

Other

PMID:

38060027

Title:

Shenghong liquid combined with nanosilver accelerates healing of foot ulcers in diabetic rats by inhibiting inflammation and increasing angiogenesis

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Cell death discovery

Application:

ELISA

IF:

7.1

Species:

Rattus norvegicus

PMID:

38378809

Title:

Galectin-3 is involved in inflammation and fibrosis in arteriogenic erectile dysfunction via the TLR4/MyD88/NF-B pathway - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Frontiers in Neurology

Application:

ELISA

IF:

2.7

Species:

Homo sapiens

PMID:

31920917

Title:

Effect of Ulinastatin Combined With Dexmedetomidine on Postoperative Cognitive Dysfunction in Patients Who Underwent Cardiac Surgery.

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Neuroscience

Application:

ELISA

IF:

2.9

Species:

Mus musculus

PMID:

38431041

Title:

Nrdp1-mediated Macrophage Phenotypic Regulation Promotes Functional Recovery in Mice with Mild Neurological Impairment after Intracerebral Hemorrhage

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Frontiers in pharmacology

Application:

ELISA

IF:

5.6

Species:

Homo sapiens

PMID:

35860030

Title:

ANGPTL4 Regulates Psoriasis via Modulating Hyperproliferation and Inflammation of Keratinocytes - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Frontiers in immunology

Application:

qPCR, ELISA

IF:

5.7

Species:

Homo sapiens

PMID:

36203605

Title:

Immunological risk factors for sepsis-associated delirium and mortality in ICU patients - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Science advances

Application:

ELISA

IF:

11.7

Species:

Homo sapiens

PMID:

37235656

Title:

PD-L1 methylation restricts PD-L1/PD-1 interactions to control cancer immune surveillance - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

ELISA

IF:

4.8

Species:

Homo sapiens

PMID:

38489235

Title:

WNT2B activates macrophages via NFB signaling pathway in inflammatory bowel disease

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

International journal of molecular sciences

Application:

ELISA

IF:

5.6

Species:

Homo sapiens

PMID:

37686220

Title:

Exosomes Derived from Hypoxia-Cultured Human Adipose Stem Cells Alleviate Articular Chondrocyte Inflammaging and Post-Traumatic Osteoarthritis Progression - PMC

References for Human TNF-alpha ELISA Kit (RK00030)

Product:

Human TNF-alpha ELISA Kit

Journal:

Open medicine (Warsaw, Poland)

Application:

ELISA

IF:

Species:

Homo sapiens

PMID:

38025528