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Rat TNF-alpha ELISA Kit (RK00029)

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ABclonal:Rat TNF-alpha ELISA Kit
ABclonal: - Rat TNF-alpha ELISA Kit (RK00029)

Overview

Product nameRat TNF-alpha ELISA Kit
Catalog No.RK00029
Size96 T
Sample100 ul
InstrumentMicroplate Reader
DetectionColorimetric
Assay Time4 hours
ConjugateBiotin
Assay TypeSandwich ELISA
Sample typeSerum, plasma and cell culture supernates
Cross reacts withRat
Product overview The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in Serum, plasma and cell culture supernates
Intended useThis Rat TNF-alpha ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Rat TNF-alpha in the sample, this Rat TNF-alpha ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Rat TNF-alpha concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage instructions Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Sensitivity15.2 pg/mL
Standard curve range31.2-2000pg/ml
ComponentsMicrotiter Plate
Enzyme Conjugate
Standards
Substrate A
Substrate B
Stop Solution
Wash Solution
Balance Solution
Safety notes1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal.
2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents.

* This package insert must be read in its entirety before using this product.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RK00029? Please let us know so that we can cite the reference in this datasheet.

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