| Product name | SARS-CoV-2 Spike RBD Protein IgG Antibody ELISA Kit |
|---|---|
| Catalog No. | RK04137 |
| Size | 96 T |
| Sample | 100 ul |
| Instrument | Microplate Reader |
| Detection | Colorimetric |
| Assay Time | 3.5 hours |
| Conjugate | HRP |
| Assay Type | Indirect ELISA |
| Sample type | serum, plasma |
|---|---|
| Cross reacts with | |
| Product overview | The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in serum, plasma (EDTA, citrate, ). |
| Intended use | This assay employs the Indirect immunoassay technique.A Spike RBD specific for Spike RBD Antibody has been pre-coated onto a microplate.Antibody and samples are pipetted into the wells and any Spike RBD Antibody present is bound by the immobilized protein. Following incubation unbound samples are removed during a wash step, and then a secondary antibody is added to the wells and binds to the combination of capture protein-Spike RBD Antibody in sample. Following a wash to remove any unbound combination, a substrate is added.A colored product TMB is formed in proportion to the amount of Spike RBD Antibody present in the sample. The reaction is terminated by addition of acid and absorbance is measured. |
| Storage instructions | Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month. |
| Components | Antigen Coated Plate Control Antibody (4000×) Concentrated Secondary Antibody (500×) Control/Sample Diluent (R1) Secondary Antibody Diluent (R2) Wash Buffer(20x) TMB Substrate Stop Solution Plate Sealers Specification |
| Safety notes | 1. This kit contains materials with small quantities of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide accumulation. Avoid ingestion and contact with eyes, skin or mucous membranes. In the case of contact, rinse the affected area with plenty of water. Observe all federal, state and local regulations for disposal. 2. All blood components and biological materials should be handled as potentially hazardous. Follow universal precautions as established by the Centers for Disease Control and Prevention and by the Occupational Safety and Health Administration when handling and disposing of infectious agents. |
* This package insert must be read in its entirety before using this product.
* For research use only. Not for therapeutic or diagnostic purposes.
