SYBR® Green Fast qPCR Mix supplied in a 2X concentration is a convenient premix designed for the rapid and sensitive real-time qPCR using SYBR® Green I dye. Genomic DNA or cDNA of interest can be quantified with this product. The SYBR® Green I dye is used to detect DNA amplicon by binding to the double-stranded DNA. The increase in fluorescent intensity only results from DNA-bound SYBR® Green I dye and is proportional to the amount of the double-stranded PCR products formed during PCR cycles. The master mix contains Taq DNA polymerase, SYBR® Green I dye, dNTP, Mg2+, and optimized buffer components. ROXTM reference dye is included in various concentrations depending on the qPCR instruments. Only template, primers and water need to be added in the qPCR reaction. ABclonal 2X SYBR Green Fast qPCR Mix exhibits stable performance with high sensitivity and specificity. The ready-to-used premix cocktail facilitates the minimization of system error, handling time and cross contaminations.
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