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ABScript II One Step SYBR Green RT-qPCR Kit (RK20404)

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Cat. No.ConcentrationSpecificationPrice
RK2040420 μL / RXN250 RXN₩360,000
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RK2040420 μL / RXN25 RXN₩48,000
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Description

ABScript II One Step SYBR Green RT-qPCR Kit is a special kit for one-step RT-qPCR reaction by using SYBR Green I chimeric fluorescence. The kit takes RNA as the template, uses gene-specific primers, and reverse transcription and PCR reaction can be carried out continuously in the same tube without additional pipe opening and pipetting operations, greatly improving the detection flux and effectively preventing contamination. This reaction system can detect the amplification products in real time, greatly improving the detection sensitivity, and omitting the electrophoresis step after PCR reaction, which is very suitable for the detection of RNA virus and other trace RNA. This product integrates the superiority of ABScript II Reverse Transcriptase and Taq DNA Polymerase, cooperate with optimized buffer system, with high amplification efficiency and high amplification specificity, one-step RT - qPCR reaction can be stable. In addition, all the enzymes used in the reaction are made into enzyme Mix, which is easier and more convenient to operate.
Product Features

  • Reverse transcription and qPCR are operated in the same reaction tube, which simplifies the experimental operation and reduces the risk of contamination.
  • The results are reliable: the recombinant reverse transcriptase reduced the activity of RNase H, improved the thermal stability and cDNA synthesis efficiency, and the hot-started Taq DNA Polymerase increased the specificity of PCR amplification.
  • High sensitivity: the optimized Buffer system greatly improved the amplification efficiency of PCR reaction, and the detection sensitivity was up to 0.1pg total RNA.
Applications

real-time PCR
Storage

Store at -20℃

Product Information

Product Components

Components25 RXN
(20 μL/ RXN)
250 RXN
(20 μL / RXN)
2X One Step SYBR Green RT-qPCR Buffer250 μL1.25 mL X 2
ABScript II One Step Enzyme Mix20 μL200 μL
50X ROX Dye I10 μL100 μL
50X ROX Dye II10 μL100 μL
Nuclease-free H2O1.25 mL1.25 mL X 2

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