ABScript Neo RT Master Mix for qPCR is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)20VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.
Product Features
Universality: It has a more stable reverse transcription efficiency for genes with different abundance and GC content;
Inhibitor tolerance: Common inhibitors introduced in RNA extraction, such as salt ions, ethanol, phenols, and SDS, have strong tolerance and better tolerance to low-quality samples.
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