ABScript Neo RT Master Mix for qPCR with gDNA Remover is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
Product Features
Convenient operation: gDNA removal and reverse transcription can be completed in one preparation, and the entire experimental process can be completed within 20 minutes;
gDNA removal: It can effectively remove gDNA from the sample without affecting reverse transcription efficiency;
Universality: It has a more stable reverse transcription efficiency for genes with different abundance and GC content;
Inhibitor tolerance: Common inhibitors introduced in RNA extraction, such as salt ions, ethanol, phenols, and SDS, have strong tolerance and better tolerance to low-quality samples.
Applications
RT-PCR
Storage
Store at -20℃
Product Information
Product Components
Components
catalog
20 RXN
100 RXN
4X ABScript Neo RT Master Mix*
RM21486
100 μL
500 μL
20X gDNA Remover Mix
RM21479
20 μL
100 μL
Nuclease-free ddH2O
RM20214
1.25 mL
1.25 mL X 2
Product Data
1. Excellent reverse transcription efficiency and universality
Use RK20433 and an imported brand to reverse transcription 1 μg human K562 cell RNA and qPCR detection of 10 genes with different expression abundances.The results showed that there was high consistency between RK20433 and imported brands for high and middle abundance genes, but for low abundance genes, the Ct value of RK20433 was lower, and one gene, RK20433, was able to detect signals, while imported brands were unable to detect signals
2. Wide Sample starting quantity compatibility
log
3
2
1
0
-1
-2
-3
E
R2
rGAPDH
17.116
19.05
22.736
26.315
29.99
32.46
36.19
96.51%
0.9954
mβ-actin
15.34
17.74
20.91
24.62
28.32
31.60
35.35
92.55%
0.9992
Rat liver samples - RNA input amounts of 1000 ng, 100 ng, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, H2O; Mouse liver sample - RNA input amounts of 1000 ng, 100 ng, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, H2O Rat and Mouse liver tissue samples were subjected to 7 10-fold gradient dilutions of RNA, and reverse transcription and qPCR detection were performed using the gradient diluted RNA as a template. The results showed that in RK20433 can maintain extremely high reverse transcription efficiency within the concentration range of 1 μg-1 pg
1. The effect of gDNA Remover on reverse transcription efficiency
To detect the effect of gDNA Remover on reverse transcription efficiency, reverse transcription of human blood samples and rat tissue samples RNA was performed with RM21485 (reverse transcription component in RK20433, corresponding to the black amplification curve in the figure) and RM21486+RM21483 (reverse transcription component in RK20433+gDNA Remover component, corresponding to the red amplification curve in the figure), In a 20 μL system, 2 μL of 100 ng/μL was added for reverse transcription, and the product cDNA was diluted in a 10 fold gradient: 1X, 0.1X, 0.01X, 0001X, 0.0001X, H2O. The results showed that adding or not adding gDNA Remover components had no effect on downstream experimental results.
Effects of dietary supplementation of different levels of gamma-aminobutyric acid on reproductive performance, glucose intolerance and placental development of gilts
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