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ABScript miRNA First-Strand Synthesis Kit (by tailing A) (RK30170)

Publication (3) Datasheet

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RK3017020 RXN₩307,500
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Description

This kit is suitable for cDNA first strand synthesis using microRNA as template through the tail addition method, where the Poly (A) tail addition reaction and reverse transcription reaction at the 3 'end of miRNA can be efficiently carried out simultaneously. ABScript miRNA-A Enzyme Mix contains Poly (A) Polymerase (PAP) and reverse transcriptase. PAP is mainly used to add Poly (A) tails at the 3 'end of RNA molecules, and can also specifically recognize single stranded RNA, effectively avoiding RT reactions of pre-miRNA with double stranded or stem-loop structures. The modified reverse transcriptase lacks of RNase H activity and increases its affinity with RNA, resulting in a significant improvement in the efficiency and sensitivity of miRNA reverse transcription. The obtained cDNA can be directly used for qPCR detection using either SYBR Green dye-base or Taqman probe-base reagent.
Product Features

  • High specificity: The kit only performs Poly (A) tail addition reaction and reverse transcription reaction on single-stranded miRNAs, avoiding interference from pre-miRNAs with secondary structure;
  • Convenient and fast: Poly (A) tail addition reaction and reverse transcription reaction can be completed in one preparation.
  • High sensitivity: Total RNA as low as 10 pg can be detected;
Applications

Poly(A) tailing and Reverse transcription of miRNA.
Storage

This kit is stable for 12 months when stored at -20℃.

Product Information

Product Components

ComponentsComponent number20 RXN
ABScript miRNA-A Enzyme Mix (20X)RM3027120 μL
ABScript miRNA-A Reaction Buffer (2X)RM30272200 μL
Universal RT PrimerRM3027360 μL
Universal miRNA-A qPCR Primer R (10 μM)RM30274200 μL
U6 qPCR Primer F (10 μM)RM30275100 μL
Nuclease-free ddH2ORM302761 mL
Product Data

Reverse transcription and qPCR detection were performed using the gradient diluted RNA as a template(1 µg/100 ng/10 ng/1 ng/100 pg/10 pg).The results showed that this kit can perform reverse transcriptional reactions on total RNA as low as 10 pg.

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