AFTSpin Multifunction DNA Purification Kit is capable of providing a simple and fast solution for gel recovery of DNA fragments and PCR product recovery, based on silica gel column purification technology. In the presence of high-disordering salts, the DNA fragments can be selectively adsorbed on the silica-based membrane in the spin column, and then the washing solution removes primers, nucleotides, proteins, enzymes, and other impurities through a series of rapid washing-centrifugation steps; the pure DNA is eluted from the silica-based membrane with a low-salt, high-pH elution buffer solution. Employing a unique gel lysis/binding solution formula, this kit integrates the two functions of gel lysis and binding as a whole, therefore one kit can be used in agarose DNA recovery, PCR product cleaning and purification, and enzyme digestion product purification and recovery. The gel lysis solution/binding solution is prepared as a yellow solution, which is convenient to observe the gel lysis effect and monitor the pH value change, thereby achieving the best binding effect and improving the recovery efficiency greatly.
Product Features
The gel lysis/binding solution is characterized by good stability and strong buffering capacity, and can buffer the pH within the optimal binding range even if the sample changes greatly;
The two functions of gel lysis and binding are integrated as a whole, therefore one kit can be used in various applications such as agarose DNA recovery, PCR product cleaning and purification, and enzyme digestion product purification and recovery;
The gel lysis/binding solution is prepared as a yellow solution, which is convenient to observe the gel lysis effect and monitor the pH value change, thereby achieving the best binding effect and improving the recovery efficiency greatly;
Fast, convenient, and free of toxic substances;
Applications
It is applicable to agarose gel DNA recovery, PCR reaction product purification and recovery, enzyme digestion product DNA fragment purification and recovery, purification and recovery after probe labeling, DNA sample concentration, etc.
Storage
This kit is stable for 12 months when stored at room temperature.The occasional particle of reagent does not affect the experimental effect.
Product Information
Product Data
Sample
1
2
3
4
5
Conc.(ng/米L)
61
12
53
61
27
Sample
1
2
3
4
5
6
Conc.(ng/米L)
35
30
26
41
24
25
RK30100 can be used for PCR products purify and gel extraction.
We use cookies to provide our customers better user experience. By continuing use this website, you acknowledge to the ABclonal Terms & Conditions and Privacy Notice.