Description
The kit is capable of lysing cells using a modified SDS-alkaline lysis method. The optimized lysate allows the silica-based membrane in the centrifugal adsorption column to selectively bind the plasmid DNA in the solution under the condition of high salt and low pH value and then removes impurities and other bacterial components by deproteinizing solution PR and washing solution WB. Finally the low-salt, high-pH eluent EB elutes the pure plasmid DNA from the silica-based membrane.
Product Features
- Easy to use: The entire experimental process can be completed in 30 minutes without the use of toxic reagents;
- High purity: deproteinizing solution PR is capable of removing residual nucleases in host cells effectively;
- Stable performance: Minimal difference in adsorption capacity between columns, and good repeatability;
Applications
The plasmid DNA extracted by the kit can be applied to various routine operations, including enzyme digestion, PCR, sequencing, ligation, transformation, library screening, in vitro translation, and transfection of some conventional passaged cells.
Storage
This kit is stable for 12 months when stored at room temperature.If the precipitate is produced when stored at 2-8°C, the solution in the kit should be placed at room temperature for a period of time before use, and if necessary, pre-heated in a water bath at 37°C for 10 minutes to dissolve the precipitate. Occasional particles in the reagent do not affect the experimental result.Prior to the first use, all RNase A from the kit should be added to Buffer P1 (final concentration 100 μg/mL) and stored at 2-8°C.
Product Data
- According to the results of agarose gel electrophoresisㄛRK30101 is better than Vendor T
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