Description
DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→5' exonuclease activity, but has lost 5'→3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5' overhangs to form blunt ends, removal of 3' overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.
Applications
Blunting, Polymerases for DNA Manipulation, DNA Sequencing , RT-PCR & cDNA Synthesis , RT-qPCR, RT-PCR and cDNA Synthesis , PCR
Storage
-20°C
Product Components
| Components | Concentration | 200 U | 1, 000 U | 5, 000 U |
| DNA Polymerase I, Large (Klenow) Fragment | 5, 000 U/mL | 40 μL | 200 μL | 1 mL |
| 10X ABuffer B | 10X | 1.25 mL | 1.25 mL | 1.25 mL X 4 |
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