Description
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated. E. coli DNA Ligase is active at a range of temperatures (4°C - 37°C).
Applications
Non-Cloning Ligation
Storage
-20°C
Product Components
| Components | 200 U | 1, 000 U |
| E.coli DNA Ligase (10, 000 U/mL) | 20 μL | 100 μL |
| 10X E.coli DNA Ligase Reaction Buffer | 50 μL | 250 μL |
Documents
