Description
Entrans 2X qPCR Probe Master Mix with UDG is a
ready-to-use reagent for probe-based qPCR
reactions, containing all components except
primers, probes and templates. This master mix
includes a chemically modified version of Taq
polymerase with Hot-start capability to inhibit nonspecific amplification, while allowing high efficiency,
sensitivity and reproducibility in qPCR amplification.
At the same time, and has joined the UDG antipollution system. The optimization of the Buffer
system allows the product to perform multiple
fluorescence quantitative experiments, and
It is suitable for multiple species and provides a
powerful tool for multi-disciplinary experimental
needs.
Product Features
- Containing UDG/dUTP anti-contamination system, effectively prevent aerosol contamination;
- Modified by chemical method with excellent specificity;
- Suitable for multiple probe detection;
- It can be stored at room temperature for at least 10 days without significant performance change.
- ABclonal nucleic acid release agent can be paired to support direct expansion
- It is suitable for the detection of African swine fever, compatible with 50min rapid amplification, and the detection sensitivity was up to 10 copies/25 ul.
Storage
Upon receipt, store all components at -20℃.
Product Components
| Components | 50 RXN
(20 μL/RXN) | 250 RXN
(20 μL/RXN) |
| Entrans 2X qPCR Probe Master Mix with UDG | 500 μL | 1.25mL X 2 |
| 50X ROX Dye I | 22μL | 100μL |
| 50X ROX Dye II | 22μL | 100μL |
Product Data
- In the application scenario of African swine fever, after RK21222 was stored at 37°C for 10 days, the P72, CD2V, and MGF gene fragments of African swine fever virus DNA were simultaneously detected by the multiple probe system, and the detection signal did not change significantly (green is -20°C, red is 37℃)
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