Description
Entrans qPCR Probe Kit is designed for probe-based qPCR reactions. It contains all components required for a rapid and reliable qPCR reaction except primers, probes and templates. When an intact probe binds to the complementary template DNA strand, the reporter dye and quencher are in proximity and the quencher absorbs the fluorescence emitted by the reporter dye. In PCR extension, the 5 '-3 'exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. This kit includes an engineered version of Taq polymerase with antibody-mediated Hot-start capability to inhibit non-specific amplification at room temperature, while allowing high efficiency, sensitivity and reproducibility in qPCR amplification. The Entrans qPCR Probe Kit is an ideal product for high-speed multiplex qPCR analyses.
Product Features
- Antibody modified hot-start Taq DNA polymerase had higher specificity.
- Quantitative accuracy;
- High expansion efficiency;
- Good repeatability;
- Wide trusted range;
- Multiple fluorescence quantitative tests can be carried out;
- Applicable to multiple species;
Applications
DNA Amplification, PCR and qPCR
Storage
Store at -20℃
Product Components
| Components | 100 RXN
(20 μL/RXN) | 500 RXN
(20 μL/RXN) |
| Entrans Taq DNA Polymerase (5, 000 U/mL) | 10 μL | 50 μL |
| 4X qPCR Probe Buffer | 500 μL | 1.25 mL X 2 |
| 50X ROX Dye I | 44 μL | 220 μL |
| 50X ROX Dye II | 44 μL | 220 μL |
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