Description
Formamidopyrimidine-DNA glycosylase (Fpg), also known as 8-oxoguanine DNA glycosylase, possesses two activities: N-glycosylase and apurinic/apyrimidinic (AP) site lyase. The former acts to excise damaged purine bases in dsDNA, creating an AP site; the latter can cleave at the 3' and 5' ends of an AP site, generating a gapped DNA molecule with 3' and 5' phosphate termini. The damaged bases primarily recognized by Fpg include: 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, formamidopyrimidine (fapy) guanine, methyl-fapy-guanine, fapy adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil.
Storage
-20°C
Product Components
| Components Name | Component number | Concentration | 500 U | 2500 U |
| Fpg | RM20510 | 8, 000 U/mL | 65 μL | 320 μL |
| 10X ABuffer A | RM20125 | 10 X | 1.25 mL | 1.25 mL × 2 |
| Recombinant Albumin (20 mg/mL) | RM20543 | 20 mg/mL | 600 μL | 600 μL |
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