Description
High Salt-T4 DNA Ligase is a salt-tolerant mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. It is designed to function at higher salt concentrations than wild type T4 DNA Ligase. The catalytic reaction requires ATP as a cofactor. High Salt-T4 DNA Ligase will join blunt end and cohesive end termini at salt concentrations as high as 300 mM without any loss in activity, and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.
Product Features
- Improves the salt tolerance of the ligation reaction.
- Enzyme activity is maintained at salt concentrations up to 300 mM.
- It can catalyze the formation of phosphodilipid bond at flat end and sticky end, and repair double-stranded DNA, RNA, DNA/RNA hybrid strand cutting.
- strict batch performance and stability quality control.
Applications
Cloning of restriction fragments
Joining linkers and adapters to blunt-ended DNA
Storage
-20°C
Product Components
| Components | 20, 000 U | 100, 000 U |
| High Salt-T4 DNA Ligase (400, 000 U/mL) | 50 μL | 250 μL |
| 10X T4 DNA Ligase Reaction Buffer | 500 μL | 500 μL |
Product Data
- The same amount of enzyme T4 DNA Ligase (ABclonal, RK21501), High Salt-T4 DNA Ligase (ABclonal, RK21506) and other Salt T4 DNA Ligase (rival product N) were used for gradient salt tolerance test (0-700 mM NaCl). The results showed that T4 DNA Ligase (ABclonal, RK21501) was rapidly inactivated under high salt conditions (> 200mM NaCl). High Salt-T4 DNA Ligase (ABclonal, RK21506) maintained more than 60% activity when the salt concentration was up to 400mM, and its tolerance to salt ion was better than its competitors.
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