| Cat. No. | Specification | Price | ||
|---|---|---|---|---|
| RK20570 | 1000 pmol | ₩798,000 | Add to Cart | |
| RK20570 | 100 pmol | ₩88,500 | Add to Cart | |
| Components | Component number | Size-1 100 pmol | Size-2 1000 pmol |
| LbCas12a Nuclease* | RM20551 | 100 pmol | 1000 pmol |
| 10X Buffer CutB | RM20105 | 1 mL | 1 mL |
LbCas12a specifically recognizes and cleaves the pUC19 sequence under the guidance of crRNA, causing double stranded breaks in the target DNA, cutting the substrate with a total length of about 2600bp into two fragments of 1750bp and 850bp.
Collect fluorescence signals using a fluorescence quantitative PCR instrument, and collect fluorescence signals every 60 seconds. LbCas12a forms a ternary complex with a specific target dsDNA under the guidance of crRNA, it will be activated for efficient trans cleavage activity against ssDNA, and the ssDNA reporter probe in the system will be fragmented to emit a fluorescence signal.
