Description
The Lyophilization One-Step RT-qPCR Probe Kit is a universal lyophilized reagent designed for RT-qPCR reactions using probes. It is suitable for lyophilization of freeze-dried powder and freeze-dried microspheres. After freeze-drying into microspheres. This kit includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, heat-labile UDG, and RNase inhibitor (RI). This kit utilizes RNA as a template and employs gene-specific primers. Reverse transcription and PCR reactions can be performed consecutively in the same tube, eliminating the need for additional tube opening or pipetting steps, significantly enhancing detection throughput. The kit utilizes dUTP/UDG for contamination prevention, with the heat-labile UDG quickly degrading U-containing contaminants at room temperature. The heat-labile UDG is rapidly deactivated at 50°C during reverse transcription, ensuring it does not affect the efficiency and sensitivity of RT-qPCR. This product contains glycerol content below 0.2% and includes lyoprotectants and excipients. It can be stored at 45°C for 6 months without degradation in performance.
Applications
One Step probe RT-qPCR, RNA viruses detection
Storage
-20°C
Product Components
| Components | Component number | Size-1
100 RXN (25 μL/RXN) | Size-2
500 RXN (25 μL/RXN) |
| Lyophilization RT Enzyme Mix | RM21489 | 10 μL | 50 μL |
| 2X Lyophilization One Step RT-qPCR Probe Mix | RM21490 | 1.25 mL | 1.25 mL*5 |
Product Data
1. Stability data
Control group: -20℃ (Red); Experimental group: RK20415 Stored at 37℃ for 7 Days (Other curves); Template: Human blood RNA (1ng/μL, 100pg/μL, 10pg/μL); Result: RK20415 product maintains its performance after 7 days at 37°C.
2. Stability Data of Lyophilized Microspheres
Control Group: -20℃ (Red); Experimental Group: Lyophilized Microspheres Stored at 45℃ for 150 Days (Other curves); Template: Human blood RNA (1ng/μL, 100pg/μL, 10pg/μL); Result: The detection of three human RNA fragments showed no significant CT shift, indicating stable performance.
Documents
